ABSTRACT
Objective To research the influence of Alismatis combined with bifendate on cytochrome P 450 in rat liver microsomes. Methods Twenty four healthy male SD rats were randomly divided into four groups:the experimental groups were given Alismatis at 140 mg·kg-1 , bifendateat 2.18 mg·kg-1 , and Alismatis plus bifendate at 140 mg+2.18 mg·kg-1 ,while the blank control group was given 0. 9% sodium chloride at 5 Ml · kg-1 . The liver microsomes were prepared upon differential centrifugation 7 days after administration.The microsomal protein concentration, cytochrome P450 content, Cytb5 content, NADPH cytochrome C reductase activity and amino pyrine N removal of methyl enzyme activity, erythromycin demethylase activity were determined by UV respectively. Results Compared with the normal control group, the combination use of Alismatis and bifendate redued the microsome content and cytochrome P450 content, while it increased the NADPH cytodrome C activity. The concentrations of cytochrome P450 were both increased by Alismatis and bifendate. Conclusion In contrast, combination ofAlismatis and bifendate reduces cytochrome P450 content which has a negative effect on phase I drug metabolism.Moreover, the combination of Alismatis and bifendate induced NADPH- cytochrome C reductase, accelerated the reduction of cytochrome P450 and inhibited cytochrome P450 isoform CYP2E1 activity.
ABSTRACT
Objective To research the influence of Alismatis combined with bifendate on cytochrome P 450 in rat liver microsomes. Methods Twenty four healthy male SD rats were randomly divided into four groups:the experimental groups were given Alismatis at 140 mg·kg-1 , bifendateat 2.18 mg·kg-1 , and Alismatis plus bifendate at 140 mg+2.18 mg·kg-1 ,while the blank control group was given 0. 9% sodium chloride at 5 Ml · kg-1 . The liver microsomes were prepared upon differential centrifugation 7 days after administration.The microsomal protein concentration, cytochrome P450 content, Cytb5 content, NADPH cytochrome C reductase activity and amino pyrine N removal of methyl enzyme activity, erythromycin demethylase activity were determined by UV respectively. Results Compared with the normal control group, the combination use of Alismatis and bifendate redued the microsome content and cytochrome P450 content, while it increased the NADPH cytodrome C activity. The concentrations of cytochrome P450 were both increased by Alismatis and bifendate. Conclusion In contrast, combination ofAlismatis and bifendate reduces cytochrome P450 content which has a negative effect on phase I drug metabolism.Moreover, the combination of Alismatis and bifendate induced NADPH- cytochrome C reductase, accelerated the reduction of cytochrome P450 and inhibited cytochrome P450 isoform CYP2E1 activity.
ABSTRACT
Notoginseng Radix et Rhizoma has the efficacy of dissolving stasis and hemostasis and reducing swelling and easing pain. Panax notoginseng saponins (PNS) is the main active component of Notoginseng Radix et Rhizoma, and the main components include ginsenoside Rb1, Rg1, Re, Rd and notoginsenoside R1. Injections with PNS as the medicinal material basis have become main TCM injections for the treatment of cerebral vascular diseases, with confirmed clinical efficacy. This article reviewed the research progress in pharmacological effects, clinical application and adverse reactions of PNS in treatment of cerebral vascular disease, with a purpose to provide references for further research and clinical application.